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Organometallics, 7:513-521, 1988. 113 M. J. S. Dewar and E. G. Zoebisch. Reactions were incubated at 37°C. After 30 minutes, fluorescence was measured in a fluorescence microplate reader using excitation at 530 ± 12.5 nm and detection at 590 ± 17.5 nm. A background of 54 fluorescence units was subtracted from each data point. Excess DNA was added (unless otherwise noted) and the samples were incubated for an additional 15 minutes at 37°C before fluorescence was measured. Furthermore, this highly selective chemiluminescent probe does not react with other activated oxygen species such as hydroxyl radical, superoxide or hydrogen peroxide.